in-vitro bioassays and chromatographic analyses used to

Recent advances of novel technologies for quality

2020-6-1Quality consistency is one of the basic attributes of medicines but it is also a difficult problem that natural medicines and their preparations must face The complex chemical composition and comprehensive pharmacological action of natural medicines make it difficult to simply apply the commonly used evaluation methods in chemical drugs It is thus urgent to explore the novel evaluation

Vol 254 No 20 Imx of October 25 pp 10102

2002-12-3incubated in vitro 90% of the melanotropic activity released represents post-a-MSH Physiologic secretion of a-MSH therefore may be related to acetylation of the hydroxy group of serine 1 Peptide IIF a previously described melanotropic Chromatographic Analyses

In vitro and in vivo peppermint (Mentha piperita)

Morphometric analyses Micropropagated plantlets and plants grown in pots were analysed after 30 d necessary to a complete plant development in vitro and for blossom initiation in pot culture Dry weights of leaves stems roots and whole plants were measured after drying plant material in a

In vitro bioassays to evaluate beneficial and adverse

2017-8-1Table 2 presents an overview of in vitro bioassays that can be used to detect possible adverse health effects The presence of possible genotoxic ingredients in botanicals and botanical extracts is an issue of particular concern given the general absence of regulatory requirements for putting botanicals and botanical preparations on the market and the fact that several botanicals are known to

Production of tropan alkaloids in the in vitro and

2014-6-16Cluster analysis was used on the basis of the dissimilarity coefficients Wild plants in vitro plants and callus cultures were divided into two broad groups (I and II) (Fig 2) Group I contained callus on line A medium and callus on line B medium and their dissimilarity coefficient was 0 348

T2144 ANALYTICAL CHARACTERIZATION OF BIOSIMILAR

2016-12-28Analyses must determine if proteins have the same biochemical biophysical and HPAEC-PAD cIEF CD and AUC which can be used to establish comparative analysis on glycoprotein products ANALYTICAL CHARACTERIZATION OF BIOSIMILAR PRODUCTS TO ESTABLISH BIOSIMILARITY – In-vitro assays – Bioassays ADCC – Cell-based assays

7 ANALYTICAL METHODS

2011-10-21In vitro radioactive strontium analyses are routinely performed in support of a personnel monitoring program or in cases where the size of an operation does not justify the cost of whole body counter facilities These analyses are usually done on urine samples but other types of body materials (e g feces or blood) may also be used

Vigor for In Vitro Culture Traits in S melongena S

Hybrids of Solanum melongena and S aethiopicum are of interest as rootstocks of eggplant as they are highly vigorous and can incorporate resistance to several diseases However hybridization between both species is difficult Therefore protocols for in vitro culture are of great interest for their micropropagation and biotechnological breeding

The in vitro control of Fusarium proliferatum by propolis

2019-8-27The in vitro control of Fusarium proliferatum by propolis457 INTRODUCTION Propolis is a gummy product produced by Apis mellifera bees during plant resin harvesting and is a form of beehive protection used to seal cracks or in embalming invaders The chemical compo-sition of propolis is complex and varies according

Natural product isolation

Covering: 2008 to 2012 Since the last comprehensive review by Otto Sticher on natural product isolation in NPR (O Sticher Nat Prod Rep 2008 25 517) a plethora of new reports on isolation of secondary compounds from higher plants marine organisms and microorganisms has been published Although methods described earlier like the liquid-solid chromatographic techniques (VLC FC MPLC

An Orthogonal Approach to Biosimilarity

2015-4-2Sadick (Catalent): As far as I am aware there is no one or even a few type(s) of assessment(s) that can verify biosimilarity It really will require an orthogonal approach that combines physicochemical and functional analyses Even for functional assays there ought to be an orthogonal array of cell-based and ELISA-based potency assessments as well as binding kinetic determinations

Chromatography and bioassays used to detect

2020-5-11The authors of the papers used an Effect Directed Analysis (EDA) approach which is a combination of chromatographic separation with in situ bioassays and physico-chemical characterisation to discover and identify bioactive compounds in complex plant samples

Mammalian toxicity testing: in vivo in vitro

2005-2-16Mammalian Toxicology Session 9 Mammalian toxicity testing: in vivo in vitro multigeneration C 2 8 9 10 20 Molecular methods high throughput testing C 9 31 The penultimate versions of projects should be submitted (via e-mail or the Web) over the next few days and all members of the class should review and comment on each of them over the next two weeks

In vitro bioassays to evaluate complex chemical

2015-9-1In total 36 different bioassays were conducted in this study () The bioassay battery encompassed five MOA classes (Escher and Leusch 2012) and were selected based on fully or partially validated for water quality assessments or to explore new pathways of potential human health concern The battery included in vitro bioassays based on mammalian bacteria and yeast cells

Genotoxic and chromatographic analyses of aqueous

ABSTRACT Peltodon longipes is used as a stimulant and emmenagogue The objective of this study was to perform genotoxic and chromatographic analyses of the extracts of two samples of P longipes collected from the cities of Santa Maria and Tupanciret RS Brazil The Allium cepa assay was used to analyze genotoxicity while high-performance liquid chromatography was employed to determine

Reference Standards for Therapeutic Proteins

2020-8-1In vitro bioassays may use a wide variety of biological formats each measuring a different functional mechanism or measuring one mechanism in different ways Differences in bioassay formats can provide different activity outputs for the same molecule A product's potency value is directly correlated with the specific bioassay used to

[Full text] Physicochemical and biological comparison

All chromatographic analyses were performed on an AKTA System (GE Healthcare Bio-Sciences Corp Piscataway NJ USA) configured for this purpose All drug substances and product batches produced by Eurofarma were comparatively evaluated by reverse-phase high-performance liquid chromatography (RP-HPLC) to determine their identity content and

7 ANALYTICAL METHODS

2011-10-21In vitro radioactive strontium analyses are routinely performed in support of a personnel monitoring program or in cases where the size of an operation does not justify the cost of whole body counter facilities These analyses are usually done on urine samples but other types of body materials (e g feces or blood) may also be used

Multiple shoot formation in Hypericum perforatum

Preliminary analysis has not shown significant differences in the hypericin levels among the in vitro shoots from the different induction treatments (data not shown) Therefore shoots from the 4 5 mmol L-1 BA + 0 05 mmol L-1 NAA were used for further chromatographic analyses

Phytochemical Fingerprinting and In Vitro Bioassays

Tectaria coadunata an ethnomedicinal fern used in Nepal to treat a large number of diseases has been poorly studied with regard to its phytochemical composition and possible bioactivity This study was performed with the aim of supporting traditional medicine as a new source of bioactive constituents Phytochemical compositions of methanol extracts were determined by nuclear magnetic

Mixture Effects of Estrogenic Pesticides at the Human

2016-1-26The used in vitro assays are suitable for the identification of estrogenic effects at the hERα and hERβ level and moreover for the hazard identification step in the risk assessment process The shown additive effects implicate that pesticide mixtures sharing a mode of action should be taken into account besides a single substance

Characterization of wastewater effluents in the

2019-6-1EBTs for the used CALUX bioassays were retrieved from the literature (Escher et al 2018 van der Oost et al 2017) and are presented in Table S2-2 (SI) The signal from each of the bioassays was compared with the EBT and thus allowed for ranking of the toxicity of wastewater effluents 2 4 Multiplex qPCR assays

Biochemical Principles and Functional Aspects of

For l-Arg an in vitro transamination product could not be identified with the employed analytical methods most likely because the highly basic nature of the guanidine group of the putative product prevents GC analyses ALD1 also showed a medium in vitro transamination activity (between 6 1% and 12 4% relative activity) toward l-Phe l-Trp l

Application Of Short

Kidney cells from control rats could rarely be passaged after the 5th week in culture (4-5 subpassages) A third in vivo—in vitro system has been used primarily to study the early stages in respiratory tract neoplasia (39 53 56 78) In these studies pellets of carcinogen in bees- wax were placed in the lumen of a tracheal transplant

Evaluation of coriander spice as a functional food by

Coriander leaves and seeds are widely used as a condiment and spice The use of roasted coriander seeds in food and beverage is very common In this study we investigated raw and roasted coriander seeds for their functional food quality using antioxidant anti-inflammatory and human tumour cell proliferation inhibitory assays

Repellency of deet and SS220 applied to skin involves

2014-5-6chromatographic analyses of the outer layer of cloth that had covered skin treated with 1 6 mmole compound/cm2 skin revealed only 0 1 nmole SS220/cm2 cloth and 2 8 nmole deet/cm2 cloth However in bioassays in which a single layer of cloth was treated with a dose of deet or SS220 equivalent to the amount